Note that sequences after bases become increasingly unreliable, and are not worth spending much time on. This changes the way the sequences are displayed. If you wish to keep them in the same order biledit they are in your directory then click on the bottom sequence file first, then click on tutorlal top one while holding the shift key. Once I am happy with that I ready to create what will become the consensus sequences. When you first install BioEdit and Chromas, the default will be that BioEdit opens the chromatogram files. Save the reverse complement as a text file under a different name. Expand the selection on a line or a square area. Repeat this process for the pstblue1vector. I check any unique differences by opening the chromatogram. Select both files with the mouse by dragging it over the file names at the left. I then select those sequences control-shift-acut control-shift-c or copy them control-a and paste them control-s to the desired BioEdit file. It is the only program I know of that allows you to edit, search and replace, and paste over the sequence title names independent of your sequences. To get the sequence of the original template strand, the Reverse Complement must be prepared.Įnter that information in the header of the MEGA file. Clicking on the opposite side, expand to the both direction. Select all the reverse sequences and cut them. Select to the end including the current residue. You may have to scroll down the program list to find it. One trick I bioedlt useful later is to always edit your sequences from the same starting base unless the starts are all messyas it makes sequence alignment much easier later. Guide to editing sequences with Chromas and BioEdit I select a point in the reverse, then select sequence to the end Edit, Select to Tutorlal, control-e. I use BioEdit to align sequences as it is free and has some handy features. I copy all the forwards to a new BioEdit file, tktorial the sequence titles Edit, Select All Sequences, control-shift-a and copy them to clipboard Edit, Copy Sequences, control-amake the new BioEdit file active and paste them in Edit, Paste Sequences, control-s. The regular copy and paste features work between copies of the program, but copying and pasting sequences does not. I always keep the BioEdit file with all forwards, reverses and consensus sequences so that if I double check stuff later it is easier to find the relevant chromatograms I can tell what sequence is from where by the sequence name. MEGA also has an alignment editor, but I’ve not really used it very much. BioEdit can also edit chromatograms, but I find Chromas to be nicer. BioEdit is a mouse-driven, easy-to-use sequence alignment editor and sequence analysis program designed and written by a graduate student. This is likely to be the final release of BioEdit. North Carolina State University, Department of Microbiology.
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